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ATCC
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Santa Cruz Biotechnology
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Image Search Results
Journal: Journal of Histochemistry and Cytochemistry
Article Title: MMTV/LTR Promoter-Driven Transgenic Expression of EpCAM Leads to the Development of Large Pancreatic Islets
doi: 10.1369/0022155415583876
Figure Lengend Snippet: MMTV-hEpCAM construct and in vitro expression. (A) Map of vector pJ5Ω with MMTV LTR-human EpCAM cDNA insert used for transgenic mice production and in vitro experiments. (B) Western blotting of hEpCAM transfected in mouse mammary carcinoma cell line (L153S). Lane 1: Control L153S cells; lane 2: L153S cells transfected with the MMTV LTR-hEpCAM construct; lane 3: MMTV LTR-hEpCAM-transfected L153S cells after 24 hr induction with 100 µM dexamethasone in the media; lane 4: immortalized human mammary cell line RC-6 expressing endogenous hEpCAM. (C) FACS analysis of control L153S cells and MMTV LTR-hEpCAM transfectants showing cell-surface translocation of hEpCAM, before (L153S/hEpCAM) and after (L153S/hEpCAM + Dex) further induction by 100 μM dexamethasone.
Article Snippet: The Human EpCAM SmaI–BglII fragment of GA733-2 cDNA (kindly provided by Dr. A. Linnenbach; Szala et al. 1990 ) was subcloned into the steroid inducible MMTV LTR promoter region of the
Techniques: Construct, In Vitro, Expressing, Plasmid Preparation, Transgenic Assay, Western Blot, Transfection, Translocation Assay